EXTRACTING CHLOROPHYLL EXPERIMENT

The experiment had been set up like a recipe as we get to “cook” a leaf in boiling water to extract its chlorophyll. We have water, methylated spirits (which is alcohol) , iodine solution, a leaf and a bunsen burner.

Firstly, we boil 150ml of water in a beaker.

Then, we cook the leaf in the 100 degrees water for 2 minutes. This is supposed to break the cell structure in the leaf so the chlorophyll will be extracted and it will flow into the water.

 

After 2 minutes, we turned off the bunsen burner and put the leaf into 20 ml of alcohol in a 50ml beaker.

The leaf was agitated till the leaf was white (mostly).

     

Finally, the leaf was put into the beaker of hot water and was later put on a tile. A few drops of iodine solution was dripped onto the leaf.

The initial green part of the leaf turned from yellowish brown to blue black after dripping iodine solution. Conclusion: the leaf photosynthesized!

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